Module 1: Degenerate PCR and Topoisomerase (TOPO)-based DNA Cloning
References
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4. The Technology Behind TOPO Cloning. Thermo Fisher Scientific. [Accessed 2023 February 7]. https://www.thermofisher.com/tw/en/home/life-science/cloning/topo/topo-resources/the-technology-behind-topo-cloning.html
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8. Making an Agarose Gel. University of Leicester. https://le.ac.uk. [Accessed 2023 February 8]. https://www.youtube.com/watch?v=wXiiTW3pflM
9. Running an Agarose Gel. University of Leicester. https://le.ac.uk. [Accessed 2023 February 8]. https://www.youtube.com/watch?v=U2-5ukpKg_Q
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11. Quick Start Protocol: GelRed® &GelGreen® Nucleic Acid Gel Stains. Biotium. [Accessed 2023 February 8]. https://biotium.com/wp-content/uploads/2013/11/PI-GelRed-GelGreen-Quick-Start-Protocol.pdf
12. Guidelines for degenerate primer design and use. Qiagen. [Accessed 2023 February 7]. https://www.qiagen.com/us/knowledge-and-support/knowledge-hub/bench-guide/pcr/introduction/guidelines-for-degenerate-primer-design-and-use
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14. QIAquick® Spin Handbook. [Accessed 2023 February 8]. https://www.qiagen.com/us/resources/download.aspx?id=95f10677-aa29-453d-a222-0e19f01ebe17&lang=en
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16. Monarch Plasmid Miniprep Kit Protocol. [Accessed 2023 February 9]. https://www.youtube.com/watch?v=7Xgy5_i6iOc
17. Tips for using the Monarch Plasmid Miniprep Kit. [Accessed 2023 February 9]. https://www.youtube.com/watch?v=Z-hDsUCLM2s